Abstract
The project will perform ecological and food web experiments with genetically modified organisms in freshwater (bacteria-green algae-zooplankton) and marine ecosystems (bacteria-mussels). Foreign DNA will be introduced by: 1. DNA transformation of bacteria, 2. by microinjection of engineered expression constructs into oocytes, 3. by letting filter-feeding zooplankton and mussels feed on genetically engineered DNA constructs, and 4. by letting zooplankton and mussels feed on powders/particles made from transgenic and unmodified maize. Post experiment monitoring will include uptake, expression and establishment of DNA in test organisms, horizontal and vertical gene transfer within the food chain, and basic ecological and life history comparisons between control organisms and organisms with different transgenic DNA fragments/sequences.
Norway