Natural and artificial Agrobacterium resistance in grape vine
Informations
- Funding country
Hungary- Acronym
- -
- URL
- -
- Start date
- 7/1/2007
- End date
- 6/30/2011
- Budget
- 31,620 EUR
Fundings
| Name | Role | Start | End | Amount |
|---|---|---|---|---|
| Thematic Programme | Grant | 7/1/2007 | 6/30/2011 | 31,620 EUR |
Abstract
Grapevine (Vitis vinifera) is affected by crown gall, caused by Agrobacterium, leading to massive damage and serious loss worldwide. The goal of our project is analyzing of the physiological, genetic and molecular basis of the resistance derived from the wild relative Vitis amurensis. Resistance reactions of a hybrid family will be characterized by inoculating them with Agrobacterium strains and DNA markers (RAPD, AFLP) linked to the resistant phenotype will be identified. To get additional data on resistant phenotypes, physiological, morphological and gene expression analyses will be performed. The role of bacterial genes in the resistance reactions will also be examined. As a second approach, inhibition of the expression of oncogenes (iaaM, iaaH) will be attempted using gene silencing technologies in plants transformed with siRNA constructs. Identification of DNA markers showing good linkage to the resistance gene will help the marker assisted selection of new Agrobacterium resistant cultivars. In a long term this will also forward the isolation of the resistance gene(s). The project has both theoretical and practical importance. Besides widening our knowledge on the phytopathological processes in grape Agrobacterium interactions it will allow us generating disease-tolerant grapevine plants using either traditional breeding or genetic engineering technologies. This work can generate publications and promote international collaborations as well.