Molecular distinction of apple cultivars and identification of molecular markers linked to resistance genes
Informations
- Funding country
Hungary- Acronym
- -
- URL
- -
- Start date
- 3/30/2006
- End date
- 12/31/2008
- Budget
- 61,247 EUR
Fundings
| Name | Role | Start | End | Amount |
|---|---|---|---|---|
| Postdoctoral Programme | Grant | 3/30/2006 | 12/31/2008 | 61,247 EUR |
Abstract
The accessibility of reliable genetic markers is essential for variety identification and/or the development of efficient breeding methods to create new apple cultivars selected for disease resistance, fruit quality or tree growth characteristics etc. The emergence of PCR-based molecular markers has created the opportunity for fine-scale genetic characterizations of germplasm collections, as well. The aim of our project is to develop the molecular distinction of Hungarian apple cultivars and breeding lines and to identify tightly linked molecular markers for disease resistance genes. Microsatellite (SSR-Simple Sequence Repeats) markers are ideal tools to provide reliable ’molecular fingerprints’ for identification and verification of apple scion cultivars. Since all individuals of the same cultivar originated from a single progenitor by vegetative propagation in apple breeding and cultivation, SSR fingerprinting can be used for cultivar differentiation. Some plant resistance genes have been already cloned and sequenced from different species. Isolation of molecular markers linked to resistance genes in apple is planned to achieve using degenerate primers designed from the highly conserved motifs of these known genes. Segregation population derived from the cross of resistance and susceptible cultivars is available for mapping of these markers.