Induction of chromosome rearrangements in wheat
Informations
- Funding country
Hungary- Acronym
- -
- URL
- -
- Start date
- 2/1/2003
- End date
- 12/31/2006
- Budget
- 23,206 EUR
Fundings
| Name | Role | Start | End | Amount |
|---|---|---|---|---|
| Thematic Programme | Grant | 2/1/2003 | 12/31/2006 | 23,206 EUR |
Abstract
The main objective of the proposal is to incorporate alien chromosome segments into the wheat genome, which can be used for gene mapping with the help of fluorescence in situ hybridization and PCR based molecular markers. The purpose of our research is to transfer the earliness, and the favorable amino acid composition of barley, the salt and drought tolerance of Aegilops biuncialis, and the disease resistance of Agropyron glael (leaf rust) into wheat. Alien chromosome segment introgressions in the wheat genome will be developed from the wheat x barley, wheat x Aegilops biuncialis, wheat x Agropyron glael hybrids, amphidiploids, addition lines which were formerly produced in Martonvásár. Chromosome rearrangements will be detected using genomic in situ hybridization (GISH) and the exact origin of the alien chromosome segments will be identified by PCR based molecular markers. The translocation breakpoints detected by GISH are physical landmarks on the chromosomes thus these help the physical mapping of the chromosomes. The agronomically useful translocation lines will be selected which could be valuable genetic materials for the wheat breeding.