Abstract
We plan to identify germline specific genes in Drosophila melanogaster with the application of a novel Gene Trapping method. In our experiments, first we will carry out a large scale mutagenesis with the gene trapping transposon and select out the insertions causing strong mutations. Most of the genes being expressed in somatic cells will be excluded from the further studies by a novel automatic system. Then, we will directly select for germline specific genes by following the expression pattern of the marker gene of the mutator element. Finally, we will determine the homozygous phenotype of homozygous viable mutations and the loss-of-function phenotype of the germline specific genes and select the ones showing germcell-less phenotype. The selected germline specific genes will be molecularly described.
Hungary