Variability and detection of Fusaria causing wheat scab
Informations
- Funding country
Hungary- Acronym
- -
- URL
- -
- Start date
- 10/1/2002
- End date
- 9/30/2003
- Budget
- 12,803 EUR
Fundings
| Name | Role | Start | End | Amount |
|---|---|---|---|---|
| Postdoctoral Programme | Grant | 10/1/2002 | 9/30/2003 | 12,803 EUR |
Abstract
One of our aims is to examine the genetic variability and population structure of Hungarian Fusarium culmorum and F. graminearum isolates using different molecular and chemical techniques (RAPD analysis, examination of mycotoxin production, presence of mycoviruses, pathogenicity, sequence analysis of known genes). Using sequence data, we would like to clarify the role of sexual reproduction, and the origin of Hungarian F. graminearum and F. culmorum populations. We also wish to examine the possible correlation between chemotypes and groupings based on sequence data. We also wish to develop and test PCR based gene probes for Fusarium species and their toxins causing Fusarium ear blight of wheat. We will develop or examine the applicability of gene probes for F. culmorum, F. graminearum, F. pseudograminearum, and the biosynthetic genes of their most important mycotoxins including DON, zearalenone (and fumonisins). In the case of F. pseudograminearum, we would like to examine the occurrence of this species in Hungary using these probes. We will examine the applicability of real-time PCR for examining wheat samples using the developed gene probes. The results would be confirmed using analytical techniques (HPLC, TLC, ELISA).