Attenuation of E. coli by global regulatory mutations
Informations
- Funding country
Hungary- Acronym
- -
- URL
- -
- Start date
- 2/1/2002
- End date
- 12/31/2005
- Budget
- 36,654 EUR
Fundings
| Name | Role | Start | End | Amount |
|---|---|---|---|---|
| Thematic Programme | Grant | 2/1/2002 | 12/31/2005 | 36,654 EUR |
Abstract
The so-called global regulators play an important role in bacterial gene expression. They simultaneously regulate the expression of several bacterial phenotypic traits including virulence factors. Investigations on the global regulatory function of leuX, rfaH, recA and rpoS loci of extra intestinal Escherichia coli isolates have both theoretical and practical importance. Detailed studies on the influence of these genes on the appearance of virulence properties may reveal if regulatory mutants could serve as the means for specific prevention. Namely, whether these regulatory mutants may provide a new approach for developing stable vaccine candidate strains which are attenuated in virulence but still capable to evoke protective immunity. The behavior of wild type strains, their mutants and transcomplementant derivatives will be studied in in vitro and in vivo models including immunization experiments. The project is a continuation of our previous studies supported by the National Research Foundation.