Adaptation and development of in situ hybridization
Informations
- Funding country
Hungary- Acronym
- -
- URL
- -
- Start date
- 2/1/2002
- End date
- 12/31/2005
- Budget
- 13,185 EUR
Fundings
| Name | Role | Start | End | Amount |
|---|---|---|---|---|
| Thematic Programme | Grant | 2/1/2002 | 12/31/2005 | 13,185 EUR |
Abstract
In recent years there has been a substantial increase in the number of plant varieties developed using various gene manipulation techniques and these are grown on even greater areas. As the number of genes isolated increases, these techniques are likely to be applied on a wider and wider scale. Genetic transformation allows the desired gene to be introduced directly into the recipient variety. A number of methods are now available for plant transformation, among which the gene gun is used most frequently for the development of transgenic wheat. Several molecular genetic techniques are available for the detection of the alien gene, one of the best of which is the molecular cytogenetic method of in situ hybridization. Compared with other molecular techniques, in situ hybridization has the advantage that it can be used to determine the exact location of the introduced gene, the number of copies and its position on the chromosome compared with other known DNA sequences.