Phylogenetic and functional diversity of fungal heme peroxidases
Informations
- Funding country
France- Acronym
- PEROXIDIV
- URL
- -
- Start date
- 1/1/2015
- End date
- -
- Budget
- 230,582 EUR
Fundings
| Name | Role | Start | End | Amount |
|---|---|---|---|---|
| AAPG - Generic call for proposals [Appel à projets générique] 2015 | Grant | 1/1/2015 | - | 230,582 EUR |
Abstract
Fungal heme peroxidases and peroxygenases are found in 3 distinct protein families. This large genetic pool represents a unique source of functional catalytic diversity, which has already being exploited in industry but needs to be further explored to foster its broader application. PeroxiDiv associates 3 partners with competences in environmental genomics, bioinformatics, biochemistry/enzymology and biotechnology to reveal, by an original approach of targeted sequence capture, the diversity of peroxidase-encoding genes expressed by fungal communities in distinct environments (grassland and forest soils, wood). This approach will allow the isolation of a large number of so-far unknown peroxidases with possibly novel biochemical properties. Their analysis in silico (phylogeny, 3D modeling) will lead to the identification of new variants that will be overexpressed and characterized with respect to their catalytic performance (substrate spectra, reaction products, stability, etc.).